Tangential Flow Microfiltration for Viral Separation and Concentration

Tangential Flow Microfiltration for Viral Separation and Concentration

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Microfluidic devices that allow biological particle separation and concentration have alphaville clothing found wide applications in medical diagnosis.Here we present a viral separation polydimethylsiloxane (PDMS) device that combines tangential flow microfiltration and affinity capture to enrich HIV virus in a single flow-through fashion.The set-up contains a filtration device and a tandem resistance channel.The filtration device consists of two parallel flow channels separated by a polycarbonate nanoporous membrane.The resistance channel, with dimensions design-guided by COMSOL simulation, controls flow permeation through the membrane in the filtration device.

A flow-dependent viral capture efficiency is observed, which likely reflects the interplay of several processes, including specific binding of target virus, physical deposition of non-specific particles, and membrane cleaning by shear flow.At the optimal flow rate, nearly 100% of viral particles in the permeate are captured on the membrane with various input viral concentrations.With its easy operation and consistent performance, redken shades eq 07m driftwood this microfluidic device provides a potential solution for HIV sample preparation in resource-limited settings.